Requirements - JRE 1.6 or greater - Main memory 2GB or greater (recommended 4GB) Installation - Unzip MSGFPlus.zip - Place MSGFPlus.jar in any folder Usage Information - Type 'java -jar MSGFPlus.jar' for command line arguments - For detailed documentation, visit http://omics.pnl.gov/software/ms-gf or https://bix-lab.ucsd.edu/pages/viewpage.action?pageId=13533355 - To convert an mzid output file into a tsv file, run 'java -cp MSGFPlus.jar edu.ucsd.msjava.ui.MzIDToTsv' Contact Information - PNNL Proteomics Staff (proteomics@pnnl.gov) - Sangtae Kim (sangtae.kim@gmail.com) Publications - MS-GF+: Universal Database Search Tool for Mass Spectrometry, Sangtae Kim, Pavel A. Pevzner, Nat Commun. 2014 Oct 31;5:5277. doi: 10.1038/ncomms6277. http://www.ncbi.nlm.nih.gov/pubmed/?term=25358478 - Spectral Probabilities and Generating Functions of Tandem Mass Spectra: A Strike against Decoy Databases, Sangtae Kim, Nitin Gupta and Pavel Pevzner, J Proteome Res. 2008 Aug;7(8):3354-63. doi: 10.1021/pr8001244. http://www.ncbi.nlm.nih.gov/pubmed/?term=18597511 Updates - http://omics.pnl.gov/software/ms-gf